Isolation of Cytoplasmic Pituitary Granules with Gonadotropic Activity
نویسندگان
چکیده
منابع مشابه
Isolation of Cytoplasmic Pituitary Granules with Gonadotropic Activity
A fraction isolated from the anterior pituitary glands of rats castrate for 8 weeks contained essentially a single cytoplasmic constituent with which the major portion of the gonadotropic hormone activity was associated. The glands were homogenized in an 0.25 M sucrose + 7.3 per cent polyvinylpyrrolidone (PVP) solution and fractionated by differential centrifugation to give a heterogeneous smal...
متن کاملHypothalamic-pituitary-gonadotropic function in girls with premature thelarche.
Hypothalamic-pituitary-gonadotropic activity was investigated in 9 girls with premature thelarche, and compared with that in 9 healthy girls and 6 girls with true precocious puberty. The gonadotropin stimulation test with luteinising hormone-releasing hormone was used. Girls with premature thelarche showed luteinising hormone response resembling that of normal girls, and follicle-stimulating ho...
متن کاملPituitary gonadotropic hormones in women with oligo/amenorrhoea.
BACKGROUND Any abnormality of menstrual cycle makes women worried and requires proper evaluation. Oligomenorhea is one of the indicators of Polycystic Disease of the Ovary (PCO) which is associated not only with reproductive failure but it also has metabolic and cardiovascular complications. The recent study was conducted to find out the role of Pituitary Gonadotropins in the diagnosis. After d...
متن کاملGonadotropic activity in pooled urine with acidification.
Since a gonadotropin-inhibiting substance in human urine is precipitated by acidification of urine, comparisons of the gonadotropic activities and the inhibitory effects in urine after acidification and removal of precipitate and in untreated urine were carried out in bioassays. Gonadotropic activity in the supernatant of acidified urine, in which a gonadotropin-inhibitor was removed through pr...
متن کاملThe Isolation and Properties of the Specific Cytoplasmic Granules of Rabbit Polymorphonuclear Leucocytes
A method has been described for isolation of the specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. Homogeneous suspensions of leucocytes were disrupted by lysis in 0.34 M sucrose. This procedure liberated the cytoplasmic contents of the cell and dissolved a considerable proportion of the nuclei. Following disruption, the sucrose lysate was separated into three fractions by d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1960
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.7.2.209